Blouin EF, Barbet AF, Kocan KM, Saliki JT. 2000. Establishment and characterization of an Oklahoma isolate of Anaplasma marginale in cultured Ixodes scapularis cells. Veterinary Parasitology, 87:301-313.

Mathew JS, Ewing SA, Panciera RJ, Kocan KM. 2000. Sporogonic development of Hepatozoon americanum (Apicomplexa) in its definitive host, Amblyomma maculatum (Acarina). J Parasitology, 85:1023-1031.

Mahan SM, Peter TF, Simbi BH, Kocan, KM, Camus, E., Barbet AF, Burridge MJ. 2000. Comparison of efficacy of American and African Amblyomma ticks as vectors of heartwater (Cowdria ruminantium) infection by molecular analyses and transmission trials. J Parasitology, 86:44-49.

Chang CC, Chomel BB, Kasten RW, Hiller R, Kocan KM, Ueno H, Yamaoto K, Bleich VC, Gonzales BJ, Swift PK, Byce WM, Jang SS, Boulouis HJ, Piemont Y. 2000. Isolation of Bartonella spp. from wild cervids, bovids and domestic cattle in North America. Emerging Infectious Diseases Journal, 6:1-11.

Lamdin JM, Howell DE, Kocan KM, Murphoey DR, Arnold DC, Fenton AW, Odell GV, Ownby CL. 2000. The venomous hair structure, venom and life cycle of Lagoa crispoata, a puss caterpillar of Oklahoma. Toxicon 38: 1163-1189,

Mathew JS, Ewing SA, Malayer JR, Fox JC, Kocan KM. 2000. Efficacy of a modified polymerase chain reaction (PCR) assay for detection of Ehrlichia canis infection. J. Vet. Diag. Invest. 12:456-459.

Kocan KM, Blouin EF, Barbet AF. 2000. Anaplasmosis control: past, present and future. Annals of the New York Academy of Science 916:501-509.

de la Fuente J, Garcia-Garcia JC, Blouin EF, Kocan KM. 2001. Differential adhesion of major surface proteins 1a and 1b of the ehrlichial cattle pathogen Anaplasma marginale to bovine erythrocytes and tick cells. J. Int. Parasitol. 31:145-153.

de la Fuente, J., van den Bussche, R. A., Kocan, K. M. 2001. Molecular phylogeny and biogeography of North American isolates of Anaplasma marginale (Rickettsiaceae: Ehrlichieae). Vet. Parasitol. 97:65-76.

Barbet AS, Jooyoung Yi, Lundgren A, McEwen BR, Blouin EF, Kocan KM. 2001. Antigenic variation of Anaplasma marginale: MSP2 diversity during cyclic transmission between ticks and cattle. Infect. Immun. 69: 3057-3066.

de la Fuente J, Kocan KM. 2001. Expression of Anaplasma marginale major surface protein 2 variants in persistently infected ticks. Infect. Immun. 69: 5151-5156.

Kocan KM. 2001. Anaplasmosis. In Service MW, Ed, Encyclopedia of Arthropod-transmitted diseases, CABI Publishing, Oxford University Press, UK, pp. 28-33.

Kocan KM, Halbur T, Blouin EF, Onet V, de la Fuente J, Garcia-Garcia J, Saliki JT. 2001. Immunization of cattle with Anaplasma marginale derived from tick cell culture. Veterinary Parasitology. 102:151-161.

de la Fuente J, Garcia-Garcia JC, Blouin EF, McEwen BR, Clawson D, Kocan KM. 2001. Major surface protein 1a effects tick infection and transmission of the ehrlichial pathogen Anaplasma marginale. Int. J. Parasitol. 31:1705-1714.

de la Fuente J, Garcia-Garcia JC, Blouin EF, Rodriguez SD, Garcia MA, Kocan KM. 2001. Evolution and function of tandem repeats in the major surface protein 1a of the ehrlichial pathogen Anaplasma marginale. Animal Health Research Reviews. 2:163-173.

Bowie MV, de la Fuente J, Kocan KM, Blouin EF, Barbet AF. 2002. Conservation of major surface protein 1 genes of the ehrlichial pathogen Anaplasma marginale during cyclic transmission between ticks and cattle. Gene 282(1-2):95-102.

de la Fuente J, Garcia-Garcia JC, Blouin EF, Kocan KM. 2002. Infection of tick cells and bovine erythrocytes with the intracellular ehrlichiae Anaplasma marginale excludes infection with other genotypes. Journal of Clinical and Diagnostic Laboratory Immunology 9:658-668.

de la Fuente, J, Van Den Bussche, RA, Garcia-Garcia JC, Rodríguez SD, García MA, Guglielmone AA, Mangold AJ, Friche Passos LM, Barbosa Ribeiro MF, Blouin EF, Kocan KM. 2002. Phylogeography of New World isolates of Anaplasma marginale based on major surface protein sequences. Vet. Microbiol. 88:275-285.

Blouin EF, Kocan KM, de la Fuente J, Saliki JT. 2002. Effect of tetracycline on development of Anaplasma marginale in cultured Ixodes scapularis cells. Vet. Parasitol. 107:115-126.

de la Fuente J, Kocan KM, Garcia-Garcia JC, Blouin EF, Claypool PL, Saliki JT. 2002. Vaccination of cattle with Anaplasma marginale derived from tick cell culture and bovine erythrocytes followed by challenge-exposure by ticks. Vet. Microbiol. 89:239-251.

Blouin EF, de la Fuente J, Garcia-Garcia JC, Sauer JR, Saliki JT, Kocan KM. 2002. Applications of a cell culture system for studying the interaction of Anaplasma marginale with tick cells. Animal Health Research Reviews. 3:57-68.

Kocan KM, de la Fuente J, Blouin EF, Garcia-Garcia JC. 2002. Adaptation of the tick-borne pathogen, Anaplasma marginale, for survival in cattle and tick hosts. In Proceedings, 4th International Conference on Ticks and Tick-borne Pathogens, Experimental and Applied Acarology, 28: 9-25.

de la Fuente J, Blouin EF, Kocan KM. 2003. Infection of ticks with the intracellular rickettsia Anaplasma marginale excludes infection with other genotypes. Clin. Diagn. Lab. Immunol. 10: 182-184.

de la Fuente J, Garcia-Garcia JC, Blouin EF, Kocan KM. 2003. Characterization of the functional domain of the major surface protein 1a involved in adhesion, infection and transmission of Anaplasma marginale. Vet. Microbiol. 91:265-283.

Blouin EF, Saliki JT, de la Fuente J, Garcia-Garcia JC, Kocan KM. 2003. Antibodies to Anaplasma marginale major surface proteins 1a and 1b inhibit infectivity for cultured tick cells. Vet Parasitol. 111: 247-260.

Almazán C, Kocan KM, Bergman DK, Garcia-Garcia JC, Blouin EF, de la Fuente J. 2003. Identification of protective antigens for control of Ixodes scapularis infestations using cDNA expression library immunization. Vaccine. 21:1492-1501.

Kocan KM, de la Fuente J. 2003. Co-feeding studies of ticks infected with Anaplasma marginale. Vet. Parasitol. 112:295-305.

de la Fuente J, Van Den Bussche, RA, Prado T, Kocan KM. 2003. Anaplasma marginale major surface protein 1a genotypes evolved under positive selection pressure but are not markers for geographic isolates. J. Clin. Microbiol. 41: 1609-1616.

Almazán C, Kocan KM, Bergman DK, Garcia-Garcia JC, Blouin EF, de la Fuente J. 2003. Characterization of genes transcribed in an Ixodes scapularis cell line that were identified by expression library immunization and analysis of expressed sequence tags. Gene Therapy and Molecular Biology, 7:43-59.

de la Fuente J, Kocan KM. 2003. Advances in the identification and characterization of protective antigens for development of recombinant vaccines against tick infestations. Expert Review of Vaccines. 2:583-593.

de la Fuente JC, Golsteyn Thomas EJ, Van Den Bussche, RA, Hamilton RG, Tanaka EE, SE Druhan, Kocan KM. 2003. Characterization of Anaplasma marginale isolated from North American bison. Applied and Environmental Microbiology 69: 5001-5005.

Almazán C, Kocan KM, Bergman DK, Garcia-Garcia JC, Blouin EF, Blas-Machado, U, Yoshioka J, de la Fuente J. 2003. Methods for the identification and characterization of tick protective antigens. In Proc. 5th International Seminar in Animal Parasitology, pp. 99-104.

Kocan KM, de la Fuente J, Guglielmone AA, Melendéz RD. 2003. Anaplasma marginale: Antigens and control alternatives for a rickettsial hemoparasite of cattle. Clinical Microbiology Reviews 16:698-712.

de la Fuente, J, Kocan KM, Garcia-Garcia JC, Blouin EF, Halbur T, Onet V. 2003. Antibodies to Anaplasma marginale major surface protein 1a reduce infectivity for ticks. J Applied Res Vet Med 1:285-292.

de la Fuente J, Garcia-Garcia JC, Barbet AF, Blouin EF, Kocan KM. 2004. Adhesion of outer membrane proteins containing tandem repeats of Anaplasma and Ehrlichia species (Rickettsiales: Anaplasmataceae) to tick cells. Vet. Micro. 98:313-322.

Garcia-Garcia JC, de la Fuente J, Kocan KM, Blouin EF, Halbur T, Onet VC, Saliki JT. 2004. Mapping of B-cell epitopes in the N-terminal repeated peptides of the Anaplasma marginale major surface protein 1a and characterization of the humoral immune response of cattle immunized with recombinant and whole organism antigens. Vet Immunol Immunopath, 98 :137-151.

Garcia-Garcia JC, de la Fuente J, Blouin EF, Halbur T, Onet V, Saliki JT, Kocan KM. 2004. Differential expression of the msp1a gene of Anaplasma marginale occurs in bovine erythrocytes and tick cells. Vet. Micro. 98:261-272.

de la Fuente J, Vicente J, Höfle U, Ruiz-Fons F, Fernández de Mera IG, Van Den Bussche RA, Kocan KM, Gortázar C. 2004. Anaplasma marginale infection in free-ranging Iberian red deer in the region of Castilla La Mancha, Spain. Vet Micro, 100:163-173.

Garcia-Garcia JC, de la Fuente J, Bell G, Blouin EF, Kocan KM. 2004. Glycosylation of major surface protein 1a and its putative role in adhesion of Anaplasma marginale to tick cells. Infect Immunol 72: 3022-3030.

Hofmann-Lehmann R, Meli ML, Dreher UM, Gönczi E, Deplazes P, Braun U, Engels, M, Schüpbach J, Jörger K, Thoma R, Griot C, Stärk K, Willi B, Schmidt, J, Kocan KM, Lutz H. 2004. Concurrent infections with vector-borne pathogens as etiology of fatal hemolytic anemia in a cattle herd from Switzerland. J Clin Micro, 42: 3775-3780.

Bokma B, Blouin EF, Bechara GH, eds, 2005. Impact of Ecological Changes on Tropical Animal Health and Disease Control. Annals NY Acad. Sci 1026.

de la Fuente J, Naranjo MV, Ruiz-Fons F, Vicente J, Estrada-Peña A, Almazán C, Kocan KM, Martín MP, Gortazar C. 2004. Prevalence of tick-borne pathogens in ixodid ticks (Acari: Ixodidae) collected from wild boar (Sus scrofa) and Iberian red deer (Cervus elaphus hispanicus) in central Spain. European Journal of Wildlife Research 50:187-196.

de la Fuente J, Passos LMF, Van Den Bussche RA, Ribeiro MFB, Facury-Filho EF, Kocan KM. 2004. Genetic diversity and molecular phylogeny of Anaplasma marginale isolates from Minas Gerais, Brazil, Veterinary Parasitology, 121: 307-316.

Kocan KM, de la Fuente JC, Golsteyn Thomas EJ, Van Den Bussche, RA, Hamilton RG, Tanaka EE, SE Druhan, Kocan KM. 2004. Characterization of Anaplasma marginale isolated from North American bison. Annals NY Acad. Sci. 1026: 114-117.

Kocan KM, Blouin EF. 2004. Dedication: Conrad Yunker. Annals NY Acad. Sci. 1026:323-324.
Coetzee JF, Apley MD, Kocan KM, Rurangirwa FR, Van Donkergoed J. 2004. Comparison of three oxytetrocycline regimes for the treatment of persistent Anaplasma marginale infections in beef cattle. Veterinary Parasitology, 127: 61-73.

Stich RW, Olah GA, Brayton KA, Brown WC, Fecheimer M, Green-Church K, Jittapalagong S, Kocan KM, McGuire TC, Rurangirwa FR, Palmer GH. 2004. Identification of a novel Anaplasma marginale appendage-associated protein that localizes with actin filaments during intraerythroctyic infection. Infect Immun, 72: 7257-7264.

de la Fuente J, Almazán C, Van Den Bussche RA, Bowman J, Yoshioka JH, Kocan KM. 2005. Characterization of genetic diversity in Dermacentor andersoni (Acari: Ixodidae) with body size and weight polymorphism. Exper. Parasitology, 109:16-20.

de la Fuente J, Massung RF, Wong SJ, Chur FK, Lutz H, Meli M, Mangold AJ, Kocan KM. 2005. Sequence analysis of the msp4 gene of Anaplasma phagocytophilum strains. J Clin Micro, 43: 1309-1317.

de la Fuente J, Ayoubi P, Blouin EF, Almazán C, Naranjo V, Kocan KM. 2005. Gene expression profiling of human promyelocytic cells in response to infection with Anaplasma phagocytophilum. Cellular Microbiology, 7: 549-559.

Almazán C, Blas-Machado U, Kocan KM, Yoshioka JH, Blouin EF, Mangold AJ, de la Fuente J. 2005. Characterization of three Ixodes scapularis cDNAs protective against tick infestations. Vaccine, 23:4403-4416.

Kocan KM, de la Fuente J, Blouin EF, Garcia-Garcia JC. 2004. Anaplasma marginale (Rickettsiales : Anaplasmataceae): recent advances in defining host-pathogen adaptations of a tick-borne rickettsia. Parasitology, 129:S285-S300.

Dreher UM, Hofmann-Lehmann R, Meli ML, Regula G, Cagienard AY, Stärk KDC, Doherr MG, Filli F, Hässig M, Braun U, Kocan KM, Lutz H. 2005 Seroprevalence of anaplasmosis among cattle in Switzerland in 1998 and 2003: No evidence of an emerging disease. Vet Microbiol. 107:71-79.

de la Fuente J, Kocan KM, Blouin EF, Almazán C, Garcia-Garcia JC. 2005. New approaches for vaccine prophylaxis of ticks and tick-borne pathogens. In Proceedings, Human and Animal Ehrlichiosis, In press.

de la Fuente, Torina A, Caracappa S, Tumino G, Furlá R, Almazán C, Kocan KM. 2005. Serologic and molecular characterization of Anaplasma species infection in farm animals and ticks from Sicily. Veterinary Parasitology, 133:357-362.

de al Fuente J, Almazán C, Blouin EF, Naranjo V, Kocan KM. 2005. RNA intereference screening in ticks for identification of protective antigens. Parasitol. Res., 96:137-141.

Revel AT, Blevins JS, Almazán C, Kocan KM, de la Fuente J, Hagman KE, Vorgard MV. 2005. bptA(bbe16) is essential for the persistence of the Lyme disease spirochete, Borrelia burgdorferi, in its natural tick vector. Proceedings of the National Academy of Science, 102:6972-6977.

de la Fuente J, Torina A, Naranjo V, Carappa S. Vicente J, Mangold AJ, Vicari D, Alongi A, Scimeca S, Kocan KM. 2005. Genetic diversity of Anaplasma marginale strains from cattle farms in the Province of Palermo, Sicily. Journal of Veterinary Medicine B, 52:226-229.

Kocan KM, Yoshioka J, Sonenshine DE, de la Fuente J, Ceraul SM, Blouin EF, Almazán C. 2005. A capillary tube feeding system for studying tick/pathogen interactions of Dermacentor variabilis (Acari: Ixodidae) and Anaplasma marginale (Rickettisales: Anaplasmataceae). Journal of Medical Entomology, 42: 864-874.

de la Fuente, J., Lew, A., Lutz, H., Meli, M.L., Hofmann-Lehmann, R., Shkap, V., Molad, T., Mangold, A.J., Almazán, C., Naranjo, V., Gortázar, C., Torina, A., Caracappa, S., García-Pérez, A.L., Barral, M., Oporto, B., Ceci, L., Carelli, G., Blouin, E.F., Kocan, K.M. 2005. Genetic diversity of Anaplasma species major surface proteins and implications for anaplasmosis serodiagnosis and vaccine development. Animal Health Research Reviews 6: 75-89.

Dreher UM, de la Fuente J, Hofmann-Lehmann R, Meli MK, Pusteria N, Kocan KM, Woldehiwet A, Regula G, Staerk KDC. 2005. Serologic cross reactivity between Anaplasma marginale and Anaplasma phagocytophilum. Clinical and Diagnostic Laboratory Immunology 12:1177-1183.

de la Fuente J, Torina A, Naranjo V, Caracappa S, Di Marco V, Alongi A, Russo M, Maggio AR, Kocan KM. 2005. Infection with Anaplasma phagocytophilum in a seronegative patient with confirmed granulocytic anaplasmosis in Sicily, Italy: Case Report. Annals Clin. Microbiol. Antimicrobials, 4:15.

Almazán C, Kocan KM, Blouin EF, de la Fuente J. 2005. Vaccination with recombinant tick antigens for the control of Ixodes scapularis adult infestations. Vaccine, 23: 5294-5298.
de la Fuente J, Ayoubi P, Blouin EF, Almazán C, Naranjo V, Kocan KM. 2006. Anaplasmosis: focusing on host-vector-pathogen interactions for vaccine development. NY Academy of Sciences, In press.

de la Fuente J, Naranjo V, Ruiz-Fons F, Höfle U, Fernández de Mera IG, Almazán C, Torina A, Kocan KM, Gortázar C. 2006. Vetebrate reservoir hosts and invertebrate vectors of human and bovine anplasmosis agents in central Spain. Vector Borne and Zoonoitc Diseases, In press.
Naranjo V, Höfle U, Vicent J, Martín MP, Ruiz-Fons F, Gortazar C., Kocan KM, de la Fuente J. 2005. Genes differentially expressed in oropharyngeal tonsils and mandibular lymph nodes of diseased and healthy European wild boar naturally exposed to Mycobacterium bovis. FEMS Immunol. Med. Microbiol., In press.

Kocan KM. 2005. Dedication: Jim C. Williams. Annals NY Acad. Sci. In press.
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